DNA Barcodes Applied to a Rapid Baseline Construction in Biodiversity Monitoring for the Conservation of Aquatic Ecosystems in the Sian Ka’an Reserve (Mexico) and Adjacent Areas

This study is focused on the aquatic environments of Sian Ka’an reserve, a World Heritage Site. We applied recently developed protocols for rapid assessment most animal taxa inhabiting any freshwater system using light traps and DNA barcodes, represented by mitochondrial gene Cytochrome Oxidase I (COI). barcoded 1037 specimens comprising mites, crustaceans, insects, fish larvae from 13 close or inside with success rate 99.8%. In total, 167 barcode index numbers (BINs) were detected. From them, we identified 43 species. All others remain as BIN. Besides, non-invasive method environmental (eDNA) to analyze adult communities sequences obtained Barcode Life Database (BOLD). round, found 25 species other terrestrial vertebrates this region. No alien was found. After comparing BINs all systems, that each water body unique respect observed. The reference library presented here represents first step future programs detect change in these ecosystems, including invasive species, improve knowledge zooplankton, enhancing task compiling barcodes not yet stored databases (such BOLD GenBank).